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    Wang, Y., Kudo, M., Xu, M., Ayub, A. and Ashraf, M.
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    Wang, Y., Guo, Y., Zhang, S. X., Wu, W. J., Wang, J., Bao, W. and Bolli,

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    All 103 studies included in the reviews evaluated at least one health outcome. Stella McCartney Contrast print sneakers shGas
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    We have categorised the studies as follows: (1) those that reported at least one positive health outcome and no negative outcomes (n = 47); (2) those that reported at least one negative health outcome and no positive outcomes (n = 14); and (3) those that report conflicting health outcomes (positive and negative) or reported little/no change in all the health outcomes measured (n = 42).

    We found no conclusive evidence that better reporting of implementation might be associated with positive health outcomes. There was a similar range of implementation scores for both the 47 studies with positive outcomes (47% scored <3, 40% scored 3 or 4, and 13% scored ⩾5) and the 42 studies with conflicting/little change in outcomes (45% scored <3, 40% scored 3 or 4, and 14% scored ⩾5). Fourteen studies reported negative outcomes, of which 84% scored <3, 15% scored 3 or 4, and none scored ⩾5 on the implementation checklists.

    We extracted textual data on implementation from all the included studies for less structured, more qualitative appraisals. However, we focus on the 14 studies with negative health outcomes.

    Implementation reporting tended to be brief and anecdotal. It was often unclear how authors had obtained their information about implementation and whether they had taken steps to avoid bias or error. These (important) objections aside, our more qualitative approach to implementation appraisal did appear to uncover potential explanations for how the implementation characteristics of some studies may have contributed to negative outcomes.

    In the participation review, we found that the only two studies with negative health outcomes evaluated participatory interventions that had been implemented in workplaces undergoing organisational downsizing. 17 We found that in the “task variety” review, negative health outcomes were more likely to result from interventions that were motivated for business reasons (managerial efficiency, productivity, cost, etc) rather than by employee health concerns. 18 However, the studies identified for the compressed working week and the shift work reviews provide evidence of positive, negative or “little change” outcomes resulting from interventions regardless of whether they were motivated by business concerns, health concerns or pressure from employees. 19 20

    Promoting effective implementation is regarded as a key stage in the design and evaluation of complex interventions, and syntheses of evidence from such evaluations should incorporate data on implementation. AVEC MODéRATION Slip j5yJ25BR
    2 We incorporated implementation data into four systematic reviews of workplace interventions, using both a specially developed checklist for measuring reporting of intervention design and implementation and a more qualitative approach to assessing such reports. We found that reporting of implementation was generally poor. Our experience led us to reflect upon whether a checklist is the best tool for appraising implementation, particularly as our qualitative approach was easier to conduct and, we conclude, more useful than the checklist-based approach.

    As described above, D 100μ s within the compartment (5.4 μm 2 /s) is 40% smaller than that in LUVs (9.4 μm 2 /s). This can be explained by the fact that the 100-μs time window is still too long to evaluate the correct diffusion rate within 230-nm compartments. DOPE is estimated to diffuse ∼60 nm in 100 μs assuming a diffusion rate of 9.4 μm 2 /s ([4 × 9.4 μm 2 /s × 10 −4 s] 1/2 ), which indicates that even at the highest frame rates, the molecule will frequently rebound from a compartment wall during a frame and hence the displacement will be frequently underestimated.

    How are the phospholipid molecules temporarily confined in these compartments? To investigate the possible involvement of extracellular domains of transmembrane proteins and the extracellular matrix due to the interaction with the attached gold particles, the cells were mildly treated with trypsin. Under the conditions in which 61% of the extracellular proteins (including extracellular domains of membrane proteins and extracellular matrix proteins) was cleaved off and 83% of heparan sulfate glycosaminoglycan was removed, the movement of DOPE molecules observed at a 25-μs resolution was not affected ( Table II ). Taken together with the fact that D 100ms is the same for both Cy3–DOPE and gold-tagged DOPE, it is concluded that the extracellular matrix and the extracellular domains of membrane proteins are not involved in the temporary corralling of phospholipids in 230-nm compartments.

    View this table:
    Table II.

    Changes of phospholipid diffusion after trypsin treatment, partial cholesterol depletion, or partial depolymerization/stabilization of f-actin

    Because DOPE, an unsaturated phospholipid, is excluded from cholesterol-enriched raft domains, the rafts may act as the diffusion obstacles for DOPE in the cell membrane. Therefore, we examined the effect of partial cholesterol depletion on DOPE diffusion ( Kilsdonk et al., 1995 ). The effect of cholesterol depletion was minimal. The diffusion rate in 230-nm compartments was slightly increased (5.4 μm 2 /s to 7.1 μm 2 /s), whereas no significant change was observed in other hop parameters ( Table II ). This result indicates that temporal corralling of DOPE is not caused by the exclusion from cholesterol-enriched raft domains.

    After latrunculin-A treatment under conditions that partially depolymerize filamentous actin ( Ayscough, 1998 ), greater compartments appeared for DOPE, the median diameter of 230-nm compartments increasing by a factor of ∼1.5 (230 nm to 340 nm), or the area by a factor of ∼2 (Table II; Fig. 4 , left). Treatment with jasplakinolide, which stabilizes f-actin ( Bubb et al., 2000 ), did not change the confinement size, but it increased the residency time by a factor of ∼2 (11 ms to 23 ms) (Table II; Fig. 4 , left), indicating that the compartment boundaries were stabilized. These results indicate that the movement of phospholipids in the outer leaf of the membrane, in spite of the lack of direct coupling with the membrane skeleton, can be regulated by the actin-based membrane skeleton fence structure.

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